Comprehensive protein sequence coverage using MagReSyn® Trypsin & MagReSyn® Chymotrypsin
نویسندگان
چکیده
The application note provides a rapid protocol and adaptable guideline for in-depth characterization of protein sequence. Digestions were performed on a four-protein mix using magnetic microparticles with immobilized proteolytic enzymes, MagReSyn® Trypsin and MagReSyn® Chymotrypsin. The orthogonal cleavage sites of Trypsin and Chymotrypsin provided complementary sets of peptides that resulted in increased sequence coverage of cumulative peptide maps. Furthermore, the digestions were rapid (1 hr, Trypsin; 0.5 hr Chymotrypsin), highly efficient, and reproducible. The method was also adapted for use with the Thermo KingfisherTM Duo magnetic microparticle handling station, in order to fully automate the sample preparation protocol for increased reproducibility in routine operation.
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